Further studies on the feasibility of one-day Salmonella detection by enzyme-linked immunosorbent assay
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چکیده
منابع مشابه
Enzyme-linked immunosorbent assay for Salmonella typhimurium in food: feasibility of 1-day Salmonella detection.
A microtitration plate, antibody-capture, enzyme-linked immunosorbent assay was developed for detection of Salmonella typhimurium. The assay utilizes a monoclonal detector antibody which shows no cross-reactions with non-Salmonella species and only a slight cross-reaction with one other Salmonella serotype. By using only one cultural stage (in a nonselective, chemically defined medium) prior to...
متن کاملDetection of Salmonella infections by polyvalent enzyme-linked immunosorbent assay.
Serum immunoglobulin G (IgG) and IgM were measured for individual Salmonella species by the enzyme-linked immunosorbent assay (ELISA). F344 rats were experimentally infected with Salmonella typhimurium (serogroup B), S. enteritidis (serogroup D), and S. rubislaw (serogroup G.) Endotoxin extracted from each serogroup served as the antigen in a classical indirect ELISA. Antibody specific for each...
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the microplate method of e nzyme-l i nke d i rnmuno s o r be n t a s s ay (elisa) , usi ng alkaline phosphatase anti-human i gg conj ugate , was modified and appl ied in t he dete c t i on of the human b lood specimens among the blood samples prep a r ed fr om human and laborat or y anima l s i n t he form of small dr ied b loodst a i ns on f ilt e r paper . the modi f i e d elisa t e chnique w...
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An enzyme-linked immunosorbent assay for neopterin using penicillinase as marker enzyme is reported here by polyclonal antibodies against neopterin conjugated to bovine serum albumin which were raised in rabbits. Immunoglobulin fractions were purified and coated on wells of microtiter plates. A chain heterology was introduced in neopterin derivative and conjugated to penicillinase. The assa...
متن کاملStandardization of an Enzyme-Linked Immunosorbent Assay for Detection of Infectious Bronchitis Virus Antibody.
An indirect enzyme–linked immunosorbent assay (ELISA) was developed for screening of antibody to avian infectious bronchitis virus (IBV). Antigen was prepared from whole-purified IBV Massachusetts serotype (BR 801 strain). Optimum dilution with minimum background for antigen concentration, rabbit anti-chicken conjugate and sera in developed ELISA was determined 0.1μg/ml, 1:3000 and 1:100, respe...
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ژورنال
عنوان ژورنال: Applied and Environmental Microbiology
سال: 1993
ISSN: 0099-2240,1098-5336
DOI: 10.1128/aem.59.5.1383-1390.1993